1. Genomic DNA is partially digested with a restriction enzyme (EcoRI). 2. The YAC is digested by the two restriction enzymes EcoRI and BamHI.3. Those two elements recombine at the EcoRI sites of YAC and are covalently linked by the DNA ligase. 4. A recombinant YAC vector, a yeast artificial chromosome with genomic DNA inserted, is produced. Then YACs vector can be introduced into yeast cells and generated an unlimited number of copies.
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